cloning dna中文是什么意思

• 克隆dna

• "clone"中文翻译    n. 1.【生物学】纯种细胞，无性系。 2.复制品；(几 ...
• "dna"中文翻译     DNA ＝ 1.deoxyribonucle ...
• "dna cloning" 中文翻译 :    dna纯系化; dna克隆化; dna克隆形成:遗传学指一特异性dna片段产身多完全相同的复制品; dna克隆形成:遗传学指一特异性dna片段产生许多完全相同的复制品; dna克篓化; dna无性繁殖; 脱氧核糖核酸克隆化
• "dna clone dna cloning" 中文翻译 :    dna克隆
• "c dna cloning" 中文翻译 :    c dna克隆
• "dna cloning vector" 中文翻译 :    dna克隆用载体; dna克隆载体
• "understanding dna and gene cloning" 中文翻译 :    dna的14堂课
• "cloning" 中文翻译 :    基因转殖 克隆; 克隆繁殖; 克隆技术; 克卢克隆化; 无性繁殖系化; 无性系化
• "cloning of" 中文翻译 :    n基因的克隆
• "expression cloning phenotype cloning" 中文翻译 :    表达克隆化
• "antibody cloning" 中文翻译 :    抗体（基因）克隆; 抗体克隆
• "car cloning" 中文翻译 :    汽车复制
• "cdna cloning" 中文翻译 :    cdna克隆化
• "clone；cloning" 中文翻译 :    基因转殖
• "cloning (programming)" 中文翻译 :    克隆 (信息学)
• "cloning and mutation" 中文翻译 :    克隆和突变
• "cloning by expression" 中文翻译 :    表达克隆
• "cloning cyndi" 中文翻译 :    克隆宠物猫
• "cloning efficiency" 中文翻译 :    克隆率; 克隆形成率; 细胞系培养效率
• "cloning equipment" 中文翻译 :    窜码器
• "cloning fidelity" 中文翻译 :    克隆保真度
• "cloning host" 中文翻译 :    克隆宿主
• "cloning screens" 中文翻译 :    克隆筛穴
• "cloning site" 中文翻译 :    插入位点,克隆位点; 克隆位点
• "cloning technique" 中文翻译 :    克隆技术

• A bt - e . coli shuttle vector pht315 was deleted its replication region of bt , then constructed a novel vector named pht315 - 1 which composed a multiple cloning site , erythromycin and ampicillin - resistance marker and could only replicated in e . coli . used pht315 - 1 , a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324 . sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501 , 333 , 183aas . orfl had 98 % identities with replicating related protein ori43 of bt strain hd263 . the others were no homology to any published bt replicating related protein . after continuous cultured for 70h at 30 c without antibiotic selecting press . the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 % . and growth curve also showed that the novel replicon was stable and could replicate normally
  进一步序列分析表明该复制区至少有3个较大的orf ，分别编码501 ， 333 ， 183个氨基酸。其中orf1蛋白序列与hd263复制蛋白ori43的同源性为98 ，而另外两个orf和genbank己公布的bt复制相关蛋白无同源性。 30连续培养72h ，复制区质粒在bt无晶体突变株hd73cry ~ -中稳定性达98以上， 30h生长曲线也表明该复制区能够在bt中稳定复制和遗传，对受体菌株无明显不良影响。
• After enzyme restriction and sequencing analysis , the nucleotide data had been further analyzed by antheprot 5 . 0 and clutalw softwares . the analysis results showed that the cloned dna fragment had a longest open reading frame ( orf ) of 1035nt , it predicted to be encoded a 344 - aa protein with the molecular weight of 36kda
  应用antheprot5 . 0 、 clustalw等分子生物学软件分析，显示主要外膜蛋白前24个氨基酸是较强的疏水性区域，可组成信号肽，其与omp基因的同源率达96 ，氨基酸的同源率高达98 。
• To prove that the cloned dna fragment can express tryptopanase , a new plasmid pet28c - tnaa , in which the cloned dna fragment was located downstream of t7 promoter on pet28c was constructed and transformed into host bl21 ( de3 ) , a bl21 lysogen of bacteriophage de3 in which the only promoter known to direct transcription of the t7 rna polymerase gene is the lacuvs promoter , which is inducible by iptg
  用iptg诱导表达t7rna聚合酶，以表达质粒上的目的基因。在葡萄糖存在的条件下，用常规方法发酵和诱导( 37 1mmiptg ) ，发现表达的蛋白质条带的分子量与理论上计算的分子量一致。但是发酵液中检测不到吲哚，表明虽然表达了目标蛋白，但表达的蛋白质没有酶活性。

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